BB 4: Rh Blood Group System

the year Karl Landsteiner and Alex Wiener discovered the Rh blood group system

ISBT number of Rh blood group system

Inheritance pattern of Rh blood group

expressor gene of D antigen

expressor gene of C, c, E, and e antigens

co-expressor of Rh antigens

nomenclature with DCEce terminology

nomenclature with Rh Hr terminology based on 8 agglutinogens

nomenclature with no genetic basis, just the presence/absence of Rh antigens

machine readable uniform nomenclature

Principle of Weak D Testing

Weak D testing is required for:

Weak D Testing procedure:

Test = _ drops of Px washed red cells + _ drops of Anti-D reagent

Weak D Testing procedure:

Control = 2 drops of ____ + 2 drops of ____

Most common Fisher-Race haplotype in Asia (70%)

cause of Genetic Weak D

cause of C Trans

cause of D Mosaic / Partial D

Weak D result:

Test = 2+ Control = 2+

Why is this result invalid?

major Rh antigen, as well as the most immunogenic

Immunogenicity of Rh antigens

True or False:

Rh antibodies activate complement.

Lacks all Rh antigens; demonstrate mild compensated hemolytic anemia

Stomatocytosis, increase in Hb F

Partial suppression of Rh gene; Features similar to Rh null but less severe

No C/c or E/e reactivity (D—/D—); Unusually strong D expression

Originally considered an allele at the C/c locus

Compound antigen; c and e are in cis position

Compound antigen; C and e are in cis position

Present on most C(+) and D(+) positive red cells

Rh Ag typing reagent that contains IgM Abs; Can be used on red cells coated with IgG Ab; CANNOT be used on Weak D testing

Rh Ag typing reagent added with bovine albumin; short incubation time; can do Weak D testing

Rh Ag typing reagent that contains IgG; Low protein-based; replaced Saline-reactive reagent

Rh Ag typing reagent derived from single clone of plasma cell containing both IgM/IgG