Microbiology test 2

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Bacteria

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83 Terms

1

Bacteria

Most well-known prokaryotes, divided into many phyla

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Proteobacteria

Gram-negative bacteria, largest phylum, includes E. coli and Salmonella

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3

Gram-positive bacteria

Distinguished by purple gram staining, large peptidoglycan component

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4

Metabolic diversity

Variety in energy and carbon sources

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5

Environmental diversity

Varied by temperature, pH, salinity, pressure, and oxygen levels

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6

Quorum sensing

Bacteria communication to sense population and environment

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7

Specialization

Differentiation in cells like spore formation for survival

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8

Cyanobacteria

Oxygenic phototrophs, form filaments, have heterocysts

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9

Spirochetes

Pathogens causing syphilis and Lyme disease

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10

Chlamydia

Respiratory and sexually transmitted intracellular pathogens

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11

Microbial Eukarya

Includes protists, fungi, and slime molds

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12

Protists

Algae and protozoa with chloroplasts, simple nutritional needs

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13

Fungi

Non-photosynthetic, crucial for nutrient recycling

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14

Viruses

Parasitic, require host for replication, contain DNA or RNA

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15

Prions

Misfolded proteins causing diseases like mad cow disease

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16

Beneficial microorganisms

Essential for recycling, food production, and biotechnology

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17

Pathogens

Microorganisms causing or capable of causing disease

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18

Gram staining

Distinguishes bacteria based on cell wall composition

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19

Peptidoglycan

Polymer in cell walls composed of NAG and NAM

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20

Cell envelope

Structural layer of bacteria including cell wall and membrane

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21

Lipopolysaccharide

Major component of Gram-negative outer membrane

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22

Bacilli

Rod-shaped bacteria

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23

Cocci

Spherical bacteria

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24

Spiral bacteria

Bacteria with a spiral shape

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25

Binomial nomenclature

Genus and species naming system for all living organisms

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26

Lipid

Non-typical phospholipid in the outer membrane

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27

Core polysaccharide

Attached to NAG in the outer membrane

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28

Fatty acid chains

Medium- and long-chain FA (C6, C12, C14, C16, C18)

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Outer Membrane

Composed of LPS layer and inner phospholipid bilayer with embedded proteins

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30

Lipoproteins

Proteins with a lipid component anchoring the outer membrane to peptidoglycan

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Structural rigidity

Function of outer membrane providing stability

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32

Endotoxin

Toxic component often referred to as Lipid A in the outer membrane

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33

Biofilms

Formed by polysaccharides aiding in attachment to surfaces

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34

Fimbriae

Filamentous structures aiding in attachment to surfaces

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35

Pili

Longer than fimbriae, aiding in attachment, conjugation, and invasion

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36

Type IV Pili

Special class aiding in attachment and twitching motility

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37

Subcellular storage compartments

Stores nutrients for survival off excess; carbon, sulfur, magnetic, polyphosphate

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38

Gas vesicles

Found in planktonic prokaryotes for buoyancy and response to cues

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39

Endospores

Dormant phase structures formed under unfavorable conditions

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40

Spore Structure

Consists of exosporium, spore coat, cortex, and core wall

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41

Appendages

Include pili and flagella for attachment and motility

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42

Motility

Three types: swimming, gliding, twitching, responding to environmental cues

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43

Bacterial Flagella

Long, thin appendages for movement and attachment

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44

Flagellar Motor

Anchored in cytoplasmic membrane and cell wall, with different rings in Gram-negative and Gram-positive bacteria

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Flagellar Speed

Rotates up to 300 rotations/sec, driven by pmf

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Swim Patterns

Differ based on flagellum location and arrangement

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Gliding Motility

Movement without flagella, slower and constant

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48

Microbial Taxes

Responses to physical or chemical stimuli, like chemotaxis and phototaxis

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49

Binary Fission

Bacterial cell division process resulting in two identical daughter cells

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50

Generation time

Time taken for a population to double in number through binary fission/Time for one cell to divide into two identical cells

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Balanced Growth

Cellular constituents increase proportionately during generation time

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Exponential Growth

Cell number doubles over a constant time interval

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54

Logarithmic Growth

Cell number plotted on a semilogarithmic scale against time

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55

Arithmetic Growth

Cell number plotted against time on a linear graph

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56

Stationary Phase

Phase where no net increase or decrease in cell population occurs

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57

Death Phase

Stage where more cells die than divide, net decrease in population

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58

Quorum Sensing

Bacteria produce virulence factors based on cell concentration

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59

Chemostat

Device to maintain constant cell population by replenishing nutrients

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60

Viable Counts

Counting live cells on a nutrient agar plate to estimate density

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61

Turbidimetric Methods

Measure turbidity to estimate cell density, quick and inexpensive

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62

Gene

Functional unit of genetic information

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63

DNA

Genetic material consisting of deoxyribonucleic acid

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64

RNA

Ribonucleic acid; expressed from DNA

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65

Double Helix Structure

Formed by complementary strands binding in a double helix

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Nucleic Acid

Consists of nucleotides with sugar, base, and phosphate

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67

Polynucleotides

Formed by phosphodiester bond formation between nucleotides

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68

mRNA

Messenger RNA carrying genetic information from DNA to ribosome

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tRNA

Transfers RNA converting mRNA information into amino acids

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70

rRNA

Ribosomal RNA, catalytic and structural components of ribosome

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Replication

Duplication of DNA double helix

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Transcription

Transfer of information from DNA to mRNA

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Translation

Synthesis of protein from mRNA, tRNA, and ribosome

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74

Whooping cough

The causation agent is the gram-negative cocci bacterium Bordetella pertussis, an infection of the respiratory tract that results in inflammation of the lungs and inability to clear the organism

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75

Polar flagellation

flagellum at one or both ends of cell

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76

Lophotrichous

numerous filaments as a tuft

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77

Amphitrichous

single tuft at opposite ends

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78

peritrichous

flagellum all over the cell

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79

eukarya types

protist, protazoans, fungi, viruse, prions

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80

Aerotaxis

response to oxygen levels

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81

Osmotaxis

response to ionic strength

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82

Protist

Include algae and protozoa, have chloroplast

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83

protozoans

Lack cell wall; majority are motile ( flagellum/cilia)

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