Cells and microscope study guide

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Robert Hooke

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Biology

10th

64 Terms

1

Robert Hooke

-First to describe cells -viewed thin slices of cork and compared them to cells in a monastery

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2

Anton van Leeuwenhoek

-Observed cell organisms -"Little animalcules" -First to observe a red blood cell and a sperm cell

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3

Robert Brown

Named the nucleus

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4

A compound light microscope

A.) Two lenses (ocular and objective) B.) Specimen must be transparent

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5

Ocular

-The eye piece -Magnifies the image

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6

Body Tube

Holds the lenses

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7

Nosepiece

rotates the objectives/lenses

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8

Stage

The platform for the slide

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9

Stage Clips

holds slide into place

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10

Diaphragm

Controls the amount of light passing through the specimen

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11

Mirror/light source

  • lights the specimen so its easier to observe

  • (Mirror reverses the image of the specimen)

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12

Arm

Used to carry the microscope

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13

Base

Supports the microscope

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14

Coarse Adjustment Knob

  • Used for preliminary focusing

  • NEVER used on high power

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15

Fine Adjustment Knob

used to focus on high power, and to fine tune low power

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16

High power objective has a magnification of 10

This objective has a magnification of 100x (this is the max magnification)

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17

Low power objective

This objective has a magnification of 10x (Minimum magnification) (Scanning objective - has a magnification of 4x)

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18

Magnification power is equal to

the product of the ocular lens times the objective

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19

resolution

Ability to distinguish between two objects very close together

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20

what limits the resolving power of the light microscope?

The properties of light

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21

electron microscope

uses a beam of electrons instead of light to get a greater resolution and magnification

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22

Dissecting microscope

used for viewing opaque specimens provides a 3-D view does not reverse the image

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23

Phase Contrast Microscope

provides contrast without stains

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24

ultracentrifuge

separates organelles by spinning them in a test tube denser parts sink to the bottom of the test tube

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25

The cell is

the basic unit of life

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26

The cell theory

  • All organisms are made up of one or more cells

  • These cells working individually, or together in tissues or organs, preform all life functions -All cells come from pre-existing cells.

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27

Exceptions to the cell theory

  • The first cell could not have come from a pre-existing cell

  • Viruses, which are NOT cells have hereditary material and can reproduce

  • Organelles such as mitochondria and chloroplasts have DNA and can reproduce

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28

prokaryote

-Do not have structures surrounded by membranes -Few internal structures -one celled organisms (like bacteria) -look like tampons

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29

eukaryote

-Contain organelles surrounded by membranes -most living organisms

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30

Cell organelles

Organelles are small specialized structures that carry out life functions within cells

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31

Plasma cell membrane

-regulates the movement of materials into or out of the cells -semi-permeable -selectively-permeable (like a door it can open and close) -composed of a lipid bilayer with embedded proteins -it is fluid meaning it is flexible and are able to move within the membrane

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32

cytoplasm

-Jelly like environment of the cell (mostly H2O)

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33

cyclosis

Movement of the cytoplasm (transports materials within the cell)

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34

Nucleus

-Controls cell activites -surrounded by nuclear membrane -contains genetic material (DNA and RNA) -Involved in reproduction

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35

Nucleolus

-Dense region in the nucleus -Involves in the synthesis of ribosomes

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36

Mitochondrion

-Site of cellular respiration -Produces energy (ATP) for metabolism

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37

Golgi Complex / Apparatus

Synthesis, packages

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38

ribosomes

Site of protein synthesis

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39

Endoplasmic Reticulum (ER)

-Forms passages throughout the cell for intracellular transport -Connects the nuclear membrane to the plasma membrane

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40

Rough ER

covered in ribosomes

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41

Smooth ER

No ribosomes

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42

lysosome

-contains digestive enzymes -breaks down damaged organelles or pathogens

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43

Vacuole

Space in a cell surrounded by a membrane

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44

Food vacuole

Stores food to be digested

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45

Contractile vacuole

helps maintain water balance within a cell

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46

Centrioles

-Only in animal cells -play a role in cell division

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47

Chloroplast

-Only in plant cells -Preform photosynthesis

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48

Cell wall

-Only in plant cells -non-living structure that surrounds, supports and protects the cell -made of cellulose (a polysaccharide)

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49

Cover slips are used for

wet mounts

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50

what is the magnification of a 20x objective

200x

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51

What substance is added to make the details of a specimen's cell structure more visible

methylene blue

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52

What is a compound microscope created for

a cell

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53

When is the field of vision largest

under low power

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54

A group of non-photosynthetic prokaryotes would all have

cell walls

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55

which two organelles work together to support animal cells

cell membrane and cytoplasm

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56

which cell structure is energy extracted from nutrients

mitochondrion

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57

what do animal cells and bacteria cells have in common

DNA and Ribosomes

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58

An ameba is a single-celled, heterotrophic organism. in order to meet its energy needs it directly relies on the interaction of which cell structures

the cell membrane and mitochondria

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59

Protein synthesis is accomplished primarily by the interaction of which two cells structures

nuclei and ribosomes

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60

The presence of which cell structure helps classify an organism as eukaryotic

nucleus

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61

One primary function of the cell membrane is

regulating the flow of simple sugars into or out of the cell

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62

A white blood cell ingests, and then digests, a number of bacteria. which cell organelles were directly responsible for the digestion of bacteria

lysosome

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63

In single-celled organisms, materials are stored primarily in

vacuoles

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64

Single-celled organisms are able to maintain homeostasis, even though they lack higher levels of organization such as organs and organ systems because

cell structures work together to maintain homeostasis in single-celled organism

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